Cascade

Cascade is designed for researchers and professionals who need high-throughput, quantitative analysis of mass spectrometry data across large-scale studies with many samples and compounds. It's ideal for routine workflows in pharmaceutical and industrial settings where speed, consistency, and automation are key. Cascade handles hundreds of thousands of samples and thousands of compounds with performance optimized for modern computing environments ^*1 .
Quantitative analysis is supported for both scan-based and SIM/SRM/MRM data using the included data conversion tools.

^*1 Processing performance depends on PC specs.

Features

Cascade offers a range of features that set it apart from other quantitative analysis tools. In addition to the main functions listed below, it also supports Excel/PDF export, cross-batch analysis, and more.

Multi-vendor Compatibility

Raw data from mass spectrometers of major manufacturers can be directly read and analyzed. Even if you have multiple mass spectrometers, including instruments for scan analysis, you can perform quantitative analysis with this single product.

This multi-vendor support is achieved by converting each raw data set to ABF or Hive format, which can be read by anyone via the API.

Supported raw data formats

Analysis Templates & Command Execution

Analysis procedures can be predefined as templates and executed via command line, without using the GUI. If the mass spectrometry system is configured to call a command after measurements, Cascade can be used to automate the analysis process. Results can be exported in CSV, TSV, Excel (.xlsx), or PDF format, and further automated using custom scripts—for example, to upload data to a database or register it in an electronic lab notebook.

Supervised Peak Detection

Conventional peak detection typically relies on fixed thresholds and predefined rules applied uniformly across all samples. Cascade’s supervised peak detection takes a different approach: it identifies similar peaks based on shape similarity to a reference peak, rather than using static parameters. It also aligns chromatograms to correct for retention time shifts, enabling robust and consistent detection even when peak shapes and timings vary.

Tutorials

To learn how to use Cascade, please refer to the tutorials below or visit the tutorial site . For complete instructions, see the user guide .

Start Analysis

Learn how to start an analysis from raw data.

• Input raw data
• Set sample type and concentration

Set Internal Standards

Learn how to set internal standards.

• Select internal standards
• Assign to target compounds

Pre-configuration to simplify analysis

Learn how to use analysis templates.

• Set up a template
• Define processing rules

Customizing display and Excel columns

Learn how to customize displayed and exported data.

• Choose display columns
• Configure Excel output

Chromatogram & peak operation

Learn how to view chromatograms and edit peaks.

Command Processing #1

Learn how to run analyses by command.

• Prepare analysis template
• Execute with rca.exe

Plans & Licensing

Cascade is licensed per PC, and can be used by multiple users who log in to the same machine.

Currently, licenses are provided through individual contracts. Online subscription plans, including monthly and annual options, will be available soon. If you are interested in a trial, please contact us using the form below.

System Requirements

This software runs on Microsoft Windows®. For more information, please see the minimum system requirements below.

FAQs

The following are the main inquiries and answers we have received regarding Cascade. If the FAQs do not answer your question, please contact us.

Q1. How does Cascade differ from other quantitative analysis software for mass spectrometry?

Q2. Can analysis be automated using Cascade?

Q3. Can Cascade be used with scan data?

Q4. Is it possible to analyze data from multiple measurement batches together?

Q5. Can I try Cascade before purchasing?